@article{Katsamenis2022,

title = {The Reconstruction of Human Fingerprints From High-Resolution Computed Tomography Data: Feasibility Study and Associated Ethical Issues},

author = {Orestis L Katsamenis and Charles B Burson-Thomas and Philip J Basford and J Brian Pickering and Martin Browne},

url = {https://www.jmir.org/2022/11/e38650},

doi = {10.2196/38650},

year  = {2022},

date = {2022-11-01},

journal = {Journal of Medical Internet Research},

volume = {24},

number = {11},

pages = {e38650},

publisher = {JMIR Publications Inc.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Keeling2022,

title = {68Ga-Bisphosphonates for the Imaging of Extraosseous Calcification by Positron Emission Tomography},

author = {George. P. Keeling and Friedrich Baark and Orestis L. Katsamenis and Jing Xue and Philip J. Blower and Sergio Bertazzo and Rafael T. M. Rosales},

url = {https://www.biorxiv.org/content/biorxiv/early/2022/11/17/2022.11.15.516425.full.pdf},

doi = {10.1101/2022.11.15.516425},

year  = {2022},

date = {2022-11-01},

journal = {biorxiv.org},

publisher = {Cold Spring Harbor Laboratory},

abstract = {Radiolabelled bisphosphonates (BPs) and [18F]NaF (18F-fluoride) are the two types of radiotracers available to image calcium mineral in vivo (e.g. bone), yet only [18F]NaF has been widely explored for the non-invasive molecular imaging of extraosseous calcification (EC) using the highly sensitive nuclear imaging technique positron emission tomography (PET). These two radiotracers bind calcium mineral deposits via different mechanisms, with BPs chelating to calcium ions and thus being non-selective, and [18F]NaF being selective for hydroxyapatite (HAp) which is the main component of bone mineral. Taking into account that the composition of EC has been reported to include a diverse range of non-HAp calcium minerals, we hypothesised that BPs may be more sensitive for imaging EC due to their ability to bind to both HAp and non-HAp deposits. To test this hypothesis, we report a comparison between the 68Ga-labelled BP tracer [68Ga]Ga-THP-Pam and [18F]NaF for PET imaging in a rat model of EC that develops macro- and microcalcifications in several organs. The presence of macrocalcifications was identified using preclinical computed tomography (CT) and microcalcifications were identified using μCT-based 3D X-ray histology (XRH) on isolated organs ex vivo. The morphological and mineral analysis of individual calcified deposits was performed using scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDX). The PET imaging and ex vivo analysis results demonstrated that while both radiotracers behave similarly for bone imaging, the BP-based radiotracer [68Ga]Ga-THP-Pam was able to detect EC more sensitively in several organs in which the mineral composition departs from that of HAp. We conclude that BP-based PET radiotracers such as [68Ga]Ga-THP-Pam have a particular advantage for the sensitive imaging and early detection of EC by being able to detect a wider array of relevant calcium minerals in vivo than [18F]NaF, and should be evaluated clinically for this purpose.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Chachlioutaki2022,

title = {Electrospun Nanofiber Films Suppress Inflammation $łess$i$greater$In Vitro$łess$/i$greater$ and Eradicate Endodontic Bacterial Infection in an $łess$i$greater$E. faecalis$łess$/i$greater$-Infected $łess$i$greater$Ex Vivo$łess$/i$greater$ Human Tooth Culture Model},

author = {Konstantina Chachlioutaki and Christina Karavasili and Elisavet Adamoudi and Anestis Tsitsos and Vangelis Economou and Charis Beltes and Nikolaos Bouropoulos and Orestis L. Katsamenis and Regan Doherty and Athina Bakopoulou and Dimitrios G. Fatouros},

doi = {10.1021/acsbiomaterials.2c00150},

year  = {2022},

date = {2022-04-01},

journal = {ACS Biomaterials Science &$mathsemicolon$ Engineering},

volume = {8},

number = {5},

pages = {2096–2110},

publisher = {American Chemical Society (ACS)},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Xenikakis2022,

title = {Transdermal delivery of insulin across human skin in vitro with 3D printed hollow microneedles},

author = {Iakovos Xenikakis and Konstantinos Tsongas and Emmanouil K. Tzimtzimis and Orestis L. Katsamenis and Efterpi Demiri and Constantinos K. Zacharis and Despoina Georgiou and Eleni P. Kalogianni and Dimitrios Tzetzis and Dimitrios G. Fatouros},

doi = {10.1016/j.jddst.2021.102891},

year  = {2022},

date = {2022-01-01},

journal = {Journal of Drug Delivery Science and Technology},

volume = {67},

pages = {102891},

publisher = {Elsevier BV},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Lawson2021,

title = {Immunofluorescence-guided segmentation of three-dimensional features in micro-computed tomography datasets of human lung tissue},

author = {Matthew J. Lawson and Orestis L. Katsamenis and David Chatelet and Aiman Alzetani and Oliver Larkin and Ian Haig and Peter Lackie and Jane Warner and Philipp Schneider},

url = {https://ui.adsabs.harvard.edu/abs/2021RSOS....811067L},

doi = {10.1098/rsos.211067},

year  = {2021},

date = {2021-11-01},

journal = {Royal Society Open Science},

volume = {8},

number = {11},

pages = {211067},

abstract = {Micro-computed tomography (µCT) provides non-destructive three-dimensional (3D) imaging of soft tissue microstructures. Specific features in µCT images can be identified using correlated two-dimensional (2D) histology images allowing manual segmentation. However, this is very time-consuming and requires specialist knowledge of the tissue and imaging modalities involved. Using a custom-designed µCT system optimized for imaging unstained formalin-fixed paraffin-embedded soft tissues, we imaged human lung tissue at isotropic voxel sizes less than 10 µm. Tissue sections were stained with haematoxylin and eosin or cytokeratin 18 in columnar airway epithelial cells using immunofluorescence (IF), as an exemplar of this workflow. Novel utilization of tissue autofluorescence allowed automatic alignment of 2D microscopy images to the 3D µCT data using scripted co-registration and automated image warping algorithms. Warped IF images, which were accurately aligned with the µCT datasets, allowed 3D segmentation of immunoreactive tissue microstructures in the human lung. Blood vessels were segmented semi-automatically using the co-registered µCT datasets. Correlating 2D IF and 3D µCT data enables accurate identification, localization and segmentation of features in fixed soft lung tissue. Our novel correlative imaging workflow provides faster and more automated 3D segmentation of µCT datasets. This is applicable to the huge range of formalin-fixed paraffin-embedded tissues held in biobanks and archives.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Rossides2021,

title = {3D cyclorama for digital unrolling and visualisation of deformed tubes},

author = {Charalambos Rossides and Sylvia L. F. Pender and Philipp Schneider},

url = {https://www.nature.com/articles/s41598-021-93184-x},

doi = {10.1038/s41598-021-93184-x},

year  = {2021},

date = {2021-07-01},

journal = {Scientific Reports},

volume = {11},

number = {1},

publisher = {Springer Science and Business Media LLC},

abstract = {Colonic crypts are tubular glands that multiply through a symmetric branching process called crypt fission. During the early stages of colorectal cancer, the normal fission process is disturbed, leading to asymmetrical branching or budding. The challenging shapes of the budding crypts make it difficult to prepare paraffin sections for conventional histology, resulting in colonic cross sections with crypts that are only partially visible. To study crypt budding in situ and in three dimensions (3D), we employ X-ray micro-computed tomography to image intact colons, and a new method we developed (3D cyclorama) to digitally unroll them. Here, we present, verify and validate our ‘3D cyclorama’ method that digitally unrolls deformed tubes of non-uniform thickness. It employs principles from electrostatics to reform the tube into a series of onion-like surfaces, which are mapped onto planar panoramic views. This enables the study of features extending over several layers of the tube’s depth, demonstrated here by two case studies: (i) microvilli in the human placenta and (ii) 3D-printed adhesive films for drug delivery. Our 3D cyclorama method can provide novel insights into a wide spectrum of applications where digital unrolling or flattening is necessary, including long bones, teeth roots and ancient scrolls.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Zdora2020,

title = {X-ray phase tomography with near-field speckles for three-dimensional virtual histology},

author = {Marie-Christine Zdora and Pierre Thibault and Willy Kuo and Vincent Fernandez and Hans Deyhle and Joan Vila-Comamala and Margie P. Olbinado and Alexander Rack and Peter M. Lackie and Orestis L. Katsamenis and Matthew J. Lawson and Vartan Kurtcuoglu and Christoph Rau and Franz Pfeiffer and Irene Zanette},

url = {https://www.osapublishing.org/optica/abstract.cfm?uri=optica-7-9-1221},

doi = {10.1364/optica.399421},

year  = {2020},

date = {2020-09-01},

journal = {Optica},

volume = {7},

number = {9},

pages = {1221},

publisher = {The Optical Society},

abstract = {High-contrast, high-resolution imaging of biomedical specimens is indispensable for studying organ function and pathologies. Conventional histology, the gold standard for soft-tissue visualization, is limited by its anisotropic spatial resolution, elaborate sample preparation, and lack of quantitative image information. X-ray absorption or phase tomography have been identified as promising alternatives enabling non-destructive, distortion-free three-dimensional (3D) imaging. However, reaching sufficient contrast and resolution with a simple experimental procedure remains a major challenge. Here, we present a solution based on x-ray phase tomography through speckle-based imaging (SBI). We demonstrate on a mouse kidney that SBI delivers comprehensive 3D maps of hydrated, unstained soft tissue, revealing its microstructure and delivering quantitative tissue-density values at a density resolution of better than 2mg/cm3 and spatial resolution of better than 8 µm. We expect that SBI virtual histology will find widespread application in biomedicine and will open up new possibilities for research and histopathology.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}